High-throughput transport-of-intensity quantitative phase imaging with aberration correction
Light: Advanced Manufacturing, ISSN: 2831-4093, Vol: 5, Issue: 4, Page: 532-541
2024
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Warsaw University of Technology Researchers Report on Findings in Manufacturing (High-throughput transport-of-intensity quantitative phase imaging with aberration correction)
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Article Description
The transport of intensity equation (TIE) is a well-established phase retrieval technique that enables incoherent diffraction limit-resolution imaging and is compatible with widely available brightfield microscopy hardware. However, existing TIE methods encounter difficulties in decoupling the independent contributions of phase and aberrations to the measurements in the case of unknown pupil function. Additionally, spatially nonuniform and temporally varied aberrations dramatically degrade the imaging performance for long-term research. Hence, it remains a critical challenge to realize the high-throughput quantitative phase imaging (QPI) with aberration correction under partially coherent illumination. To address these issues, we propose a novel method for high-throughput microscopy with annular illumination, termed as transport-of-intensity QPI with aberration correction (TI-AC). By combining aberration correction and pixel super-resolution technique, TI-AC is made compatible with large pixel-size sensors to enable a broader field of view. Furthermore, it surpasses the theoretical Nyquist-Shannon sampling resolution limit, resulting in an improvement of more than two times. Experimental results demonstrate that the half-width imaging resolution can be improved to ~345 nm across a 10× field of view of 1.77 mm (0.4 NA). Given its high-throughput capability for QPI, TI-AC is expected to be adopted in biomedical fields, such as drug discovery and cancer diagnostics.
Bibliographic Details
Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences
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