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The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci

eLife, ISSN: 2050-084X, Vol: 10
2021
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Article Description

Multidrug-resistant Streptococcus pneumoniae emerge through the modification of core genome loci by interspecies homologous recombinations, and acquisition of gene cassettes. Both occurred in the otherwise contrasting histories of the antibiotic-resistant S. pneumoniae lineages PMEN3 and PMEN9. A single PMEN3 clade spread globally, evading vaccine-induced immunity through frequent serotype switching, whereas locally circulating PMEN9 clades independently gained resistance. Both lineages repeatedly integrated Tn916-type and Tn1207.1- type elements, conferring tetracycline and macrolide resistance, respectively, through homologous recombination importing sequences originating in other species. A species-wide dataset found over 100 instances of such interspecific acquisitions of resistance cassettes and flanking homologous arms. Phylodynamic analysis of the most commonly sampled Tn1207.1-type insertion in PMEN9, originating from a commensal and disrupting a competence gene, suggested its expansion across Germany was driven by a high ratio of macrolide-to-b-lactam consumption. Hence, selection from antibiotic consumption was sufficient for these atypically large recombinations to overcome species boundaries across the pneumococcal chromosome.

Bibliographic Details

Joshua C D'Aeth; Mark PG van der Linden; Lesley McGee; Herminia de Lencastre; Paul Turner; Jae-Hoon Song; Stephanie W Lo; Rebecca A Gladstone; Raquel Sá-Leão; Kwan Soo Ko; William P Hanage; Robert F Breiman; Bernard Beall; Stephen D Bentley; Nicholas J Croucher; Alejandra Corso; Diego Faccone; Paula Gagetti; Abdullah W Brooks; Md Hasanuzzaman; Roly Malaker; Samir K Saha; Alexander Davydov; Leonid Titov; Maria Cristina de Cunto Brandileone; Samanta Cristine Grassi Almeida; Margaret Ip; Pak Leung Ho; Pierra Law; Chunjiang Zhao; Hui Wang; Jeremy Keenan; Eric Sampane-Donkor; Balaji Veeraraghavan; Geetha Nagaraj; KL Ravikumar; Noga Givon-Lavi; Nurit Porat; Rachel Benisty; Ron Dagan; Godfrey Bigogo; Jennifer Verani; Anmol Kiran; Dean B Everett; Jennifer Cornick; Maaike Alaerts; Shamala Devi Sekaran; Stuart C Clarke; Houria Belabbès; Idrissa Diawara; Khalid Zerouali; Naima Elmdaghri; Benild Moiane; Betuel Sigauque; Helio Mucavele; Andrew J Pollard; Rama Kandasamy; Philip E Carter; Stephen Obaro; Sadia Shakoor; Deborah Lehmann; Rebecca Ford; Theresa J Ochoa; Anna Skoczynska; Ewa Sadowy; Waleria Hryniewicz; Sanjay Doiphode; Ekaterina Egorova; Elena Voropaeva; Yulia Urban; Metka Paragi; Tamara Kastrin; Anne Von Gottberg; Kedibone M Ndlangisa; Linda De Gouveia; Mignon Du Plessis; Mushal Ali; Nicole Wolter; Shabir A Madhi; Susan A Nzenze; Somporn Srifuengfung; Brenda Kwambana-Adams; Ebenezer Foster-Nyarko; Ebrima Bojang; Martin Antonio; Peggy-Estelle Tientcheu; Jennifer Moïsi; Michele Nurse-Lucas; Patrick E Akpaka; Özgen Köseoglu Eser; Alison Maguire; David Aanensen; Leon Bentley; Jyothish N Nair Thulasee Bhai; Rafal Mostowy; John A Lees; Keith P Klugman; Paulina Hawkins; David Cleary

eLife Sciences Publications, Ltd

Neuroscience; Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology

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