Phyto-fabrication of silver nanoparticle using leaf extracts of Aristolochia bracteolata Lam and their mosquito larvicidal potential

This research was designed to evaluate the fine quality of silver nanoparticles (AgNPs) fabricating proficiency of aqueous leaf extract of Aristolochia bracteolata and evaluate their larvicidal potential against I-IV th instar larvae and pupa of Culex quinquefasciatus. Within 24 h, the A. bracteolata aqueous leaf extract significantly reduced the AgNO 3 and yielded AgNPs. It was initially confirmed by a visible color change from pale yellow to dark reddish brown in reaction mix and under UV–vis spectrum analysis the absorbance peak related to AgNPs was found at 430 nm. The nature of the phyto-synthesized AgNPs was characterized by standard analytical techniques such as Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Energy Dispersive X-Ray analyses (EDX). The A. bracteolata synthesized AgNPs were predominantly spherical in shape and size ranging from 29.30 to 46.32 nm. The percentage of Ag elemental composition was found as 57.61 % using EDX analysis. The A. bracteolata yielded AgNPs showed significant larvicidal and pupicidal efficiency on youngster (I-IV th instar larvae and pupa) of Culex quinquefasciatus mosquito vector. These results conclude that the aqueous leaf extract of A. bracteolata can produce biologically active AgNPs.