Detection of superoxide anion using an isotopically labeled nitrone spin trap: potential biological applications
FEBS Letters, ISSN: 0014-5793, Vol: 473, Issue: 1, Page: 58-62
2000
- 147Citations
- 44Captures
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Metrics Details
- Citations147
- Citation Indexes147
- 147
- CrossRef124
- Captures44
- Readers44
- 44
Article Description
We describe the synthesis and biological applications of a novel nitrogen-15-labeled nitrone spin trap, 5-ethoxycarbonyl-5-methyl-1-pyrroline N -oxide ([ 15 N]EMPO) for detecting superoxide anion. Superoxide anion generated in xanthine/xanthine oxidase (100 nM min −1 ) and NADPH/calcium-calmodulin/nitric oxide synthase systems was readily detected using EMPO, a nitrone analog of 5,5′-dimethyl-1-pyrroline N -oxide (DMPO). Unlike DMPO-superoxide adduct (DMPO–OOH), the superoxide adduct of EMPO (EMPO–OOH) does not spontaneously decay to the corresponding hydroxyl adduct, making spectral interpretation less confounding. Although the superoxide adduct of 5-(diethoxyphosphoryl)-5-methyl-pyrroline N -oxide is more persistent than EMPO–OOH, the electron spin resonance spectra of [ 14 N]EMPO–OOH and [ 15 N]EMPO–OOH are less complex and easier to interpret. Potential uses of [ 15 N]EMPO in elucidating the mechanism of superoxide formation from nitric oxide synthases, and in ischemia/reperfusion injury are discussed.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0014579300014988; http://dx.doi.org/10.1016/s0014-5793(00)01498-8; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0034604057&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/10802059; https://febs.onlinelibrary.wiley.com/doi/10.1016/S0014-5793%2800%2901498-8; http://doi.wiley.com/10.1016/S0014-5793%2800%2901498-8; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2FS0014-5793(00)01498-8
Wiley
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