Antigenic and immunogenic properties of membrane proteins solubilized by sodium desoxycholate, papain digestion or high ionic strength
Immunochemistry, ISSN: 0019-2791, Vol: 12, Issue: 1, Page: 9-17
1975
- 24Citations
- 2Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations24
- Citation Indexes24
- 24
- CrossRef23
- Captures2
- Readers2
Article Description
Comparison between antigens solubilized from MOPC-460 mouse plasmacytoma cell membranes by means of three methods (treatment with a surfactant: sodium desoxycholate (DOC); high ionic strength, using 3 M potassium chloride (3 M KCl); insolubilized papain (PAP) digestion) is reported. DOC displayed the greatest quantitative efficiency and brought more than 80 per cent of the membrane proteins in solution, as well as about 15 per cent of the protein-bound sugars. 3 M KCl and papain solubilized 16 per cent and 10 per cent proteins and 3 per cent and 5 per cent sugars respectively. SDS-acrylamide electrophoresis showed that DOC also solubilized the greatest number of proteins and demonstrated the disadvantages associated with PAP digestion. In the rabbit, however, DOC-solubilized proteins displayed poor immunogenicity. In particular, precipitating antibodies against membrane antigens that were not equally solubilized by 3 M KCl could not be obtained. It is deduced from what is known of surfactant mechanisms that DOC solubilizes both ‘integral’ and ‘extrinsic’ membrane proteins, whereas the ionic strength acts primarily on the latter. Cross-adsorption experiments with the antisera produced against antigens solubilized with the two agents showed that the great majority of the antibodies induced against antigens solubilized from cell membranes were directed against ‘extrinsic’ proteins.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/0019279175900440; http://dx.doi.org/10.1016/0019-2791(75)90044-0; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0016426839&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/1140821; https://linkinghub.elsevier.com/retrieve/pii/0019279175900440; http://dx.doi.org/10.1016/0019-2791%2875%2990044-0
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know