Contribution of the copper ions in the dinuclear active site to the stability of Carcinus aestuarii hemocyanin
Archives of Biochemistry and Biophysics, ISSN: 0003-9861, Vol: 439, Issue: 1, Page: 42-52
2005
- 5Citations
- 5Captures
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Metrics Details
- Citations5
- Citation Indexes5
- CrossRef5
- Captures5
- Readers5
Article Description
We have investigated the effect of copper binding on the structural properties of hemocyanin (Hc). To this aim, we have studied the holo- and apo-form of the protein, both in the hexameric and in the monomeric state ( Cae SS2 subunit), with experimental approaches that report on the protein aggregation and conformational stability. The results of gel-filtration chromatography and small angle X-ray scattering (SAXS) provide evidence that the hydrodynamic and gyration radius ( R g ) of Hc in the hexameric form only slightly increase upon copper removal, whereas a remarkable enhancement in the R g value is observed for the Cae SS2 monomer. CD measurements in the far- and near-UV region indicate that removal of copper only marginally affects the conformation of the hexameric Hc. Instead, copper depletion in the Cae SS2 strongly alters the tertiary structure of the monomer (near-UV CD), even though it is almost inconsequential on the secondary structure content (far-UV CD). These findings are fully consistent with the results of limited proteolysis experiments showing that the hexameric Hc is similarly resistant to proteolysis by trypsin both in the holo- and apo-form. Conversely, the apo-form of Cae SS2 monomer is much more susceptible to proteolytic attack by trypsin than the holo-form. Based on SAXS measurements, the concentration-dependent oligomerization process for apo- Cae SS2 has been analyzed on the basis of a thermodynamic model involving a concentration-dependent equilibrium between a monomer in a native-like and an hexameric aggregate of monomers.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0003986105001888; http://dx.doi.org/10.1016/j.abb.2005.05.005; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=20444461900&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/15950169; https://linkinghub.elsevier.com/retrieve/pii/S0003986105001888; https://dx.doi.org/10.1016/j.abb.2005.05.005
Elsevier BV
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