In vivo accumulation of sulfated glycoprotein 2 mRNA in rat thymocytes upon dexamethasone-induced cell death
Biochemical and Biophysical Research Communications, ISSN: 0006-291X, Vol: 175, Issue: 3, Page: 810-815
1991
- 88Citations
- 5Captures
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Metrics Details
- Citations88
- Citation Indexes88
- 88
- CrossRef57
- Captures5
- Readers5
Article Description
Two hours after a single intraperitoneal injection of dexamethasone (20 μg/Kg b.w.) into adult male rats, a typical ladder of DNA fragments was detectable upon separation on agarose gels of DNA from thymocytes. This became maximally evident at 4 hours. Accumulation of sulfated glycoprotein-2 (SGP-2) mRNA, whose rate of expression has been associated to the processes of programmed cell death, preceded the appearance of DNA degradation, starting to increase as early as 30 min after steroid injection, and maintained higher than controls until 8 hrs; a different time course was shown by changes in the levels of beta-actin mRNA. In the spleen, under the same conditions, the SGP-2 message also increased at 30 min, prior to DNA fragmentation, but decreased thereafter below the control value.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/0006291X9191637R; http://dx.doi.org/10.1016/0006-291x(91)91637-r; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025904519&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/2025255; https://linkinghub.elsevier.com/retrieve/pii/0006291X9191637R; http://linkinghub.elsevier.com/retrieve/pii/0006291X9191637R; http://api.elsevier.com/content/article/PII:0006291X9191637R?httpAccept=text/xml; http://api.elsevier.com/content/article/PII:0006291X9191637R?httpAccept=text/plain; http://dx.doi.org/10.1016/0006-291x%2891%2991637-r; https://dx.doi.org/10.1016/0006-291x%2891%2991637-r
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