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The primary tissue culture of human adrenocortical Conn's adenomata

Pathology - Research and Practice, ISSN: 0344-0338, Vol: 173, Issue: 1, Page: 66-81
1981
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Article Description

Primary cultures of dissociated parenchymal cells from Conn's adenomata causing primary hyperaldosteronism were successfully set up by a method previously used with normal adult human and rat adrenocortical tissue. In such cultures the adenomatous cells largely prevailed (making up 87% of the whole cell population), could survive for lengthy terms (at least up to 30 days), and were endowed with a spontaneous, discrete capability to proliferate. The de novo RNA- and DNA-synthetic and mitotic activities of Conn's cells were markedly stimulated in cultures exposed between day 16 and 21 to daily doses of exogenous cyclic AMP, either alone or in equimolar association with cyclic GMP. A significantly weaker, though still prominent enhancement of adenomatous cell growth was elicited also by daily administrations of an equimolar mixture of ACTH 1-24 and angiotensin II. In contrast, little stimulation or inhibition of growth or no effect at all could be observed when cyclic GMP, ACTH 1-24, and angiotensin II were respectively administered, each by itself.

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