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Histophotometrical investigations on the content of protein and protein thiols in the epithelium and stroma of the human uterine cervix - II. Intraepithelial neoplasia

Histochemistry, ISSN: 0301-5564, Vol: 78, Issue: 1, Page: 95-100
1983
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Fresh frozen sections of the uterine cervix of patients suffering from dysplasia (16 cases) and from carcinoma in situ (8 cases) were stained with DDD-Fast-blue B for reactive protein thiols (PSH) as well as with Amido black B for proteins. The resulting extinctions were measured in a rapidly scanning microspectrophotometer at 560 nm for PSH and at 620 nm for proteins and are given as mean extinction per unit area (E/μm), as a direct and quantitative measure for the contents of PSH and proteins respectively. Epithelial PSH increase, although still insignificantly, from mild to severe dysplasia. Also the stromal PSH show an increase which, compared with the value observed with normal cases, is now significant with regard to moderate and severe dysplasia. The ratio between epithelial and stromal PSH (Q) was calculated to an average of 1.52, which differs significantly from Q determined in normal cases (2.64) in a previous paper. The ratio PSH/protein indicates a higher PSH content of both epithelial and stromal proteins compared with normal cases. This result is in accordance with the well known functional importance of PSH for the cell division which is stimulated in dysplasia. In 8 cases of CIS and also 3 cases of invasive carcinoma a remarkable decrease of the epithelial PSH was found. The mean values of E/μm differ significantly from the respective data obtained both from normal and dyplastic cases. Still more striking is the finding that also apparently normal parts of the sections show the low values of the epithelial PSH. Several possibilities exist to explain this effect which, at first sight, seems to be absolutely unexpected, but possibly might be connected with the abnormalities in the electron spin resonance signals that have been previously found. The Q obtained from samples of CIS was calculated to an average of 1.30, which differs with a high degree of significance from that observed in normal cases. © 1983 Springer-Verlag.

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