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Utilizing Transposon Mutagenesis to Identify Genetic Elements Important for Polymicrobial Interactions

2023
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Microorganisms interact in symbiotic and antagonistic relationships. These unique interactions can be used to identify novel targets and understand microbial diseases. A genetic library of A. faecalis strains was constructed by transposon mutagenesis and screened to determine the loss-of-function of A. faecalis’ inhibition of Bacillus, Candida, and Staphylococcus. These results are important in determining the genetic mechanism in which A. faecalis inhibits growth and how this information can be used to thwart common human pathogens at a clinical level.

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