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Effects of Antifibroblast Antiserum on Cells Derived from Fibroblast Outgrowth of Human Prostatic Tissues

1975
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The purpose of this investigation was to provide pure cultures of normal human prostatic epithelium free of fibroblasts in order to study malignant conversion by chemical carcinogens. Normal epithelial cells were needed because this was the cell type implicated in prostatic malignancies of human subjects. Unfortunately fibroblasts grew faster than epithelial cells so that cultures were always overgrown with connective tissue elements. It was considered important to find a method which would eliminate fibroblasts so that normal epithelial cells could grow out in pure culture.

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