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qPCR data - Mid-pregnancy poly(I:C) viral mimic disrupts placental ABC transporter expression and leads to long-term offspring motor and cognitive dysfunction

2022
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Abstract: Limited information is available about the effect of mid-pregnancy viral infections on the placental expression of efflux transporters and offspring behavior. We hypothesized that maternal exposure to polyinosinic-polycytidylic acid [poly(I:C)], a synthetic double-stranded RNA viral mimic, would impair placental cell turnover, the expression of selected ABC transporters and adult offspring behavior. C57BL/6 mice were administered poly(I:C) (10mg/Kg;ip) or vehicle at gestational day (GD) 13.5 (mid-pregnancy). Dams were euthanized for blood collection 4 h after injection, fetal and placental collection at GD18.5 or allowed to deliver spontaneously at term. At GD 13.5, poly(I:C) induced an acute pro-inflammatory response characterized by an increase in maternal plasma levels of IL-6, CXCL-1 and CCL-2. At GD 18.5, poly(I:C) decreased cell proliferation/death in the labyrinthine and increased cell death in the junctional zones, a disruption of placental cell turnover. Abca1 and...

Bibliographic Details

Monteiro, Victoria; Andrade, Cherley; Gomes, Hanailly; Reginatto, Mila; Império, Güínever; Fontes, Klaus; Spiess, Daiane; Júnior, William; Nascimento, Veronica; Lima, Camila; Sousa, Ronny; Bloise, Flavia; Matthews, Stephen; Bloise, Enrrico; Pimentel-Coelho, Pedro; Ortiga-Carvalho, Tania

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