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Sensitive, Non‐Destructive Detection and Analysis of Neoantigen‐Specific T Cell Populations from Tumors and Blood

SSRN, ISSN: 1556-5068
2018
  • 1
    Citations
  • 1,358
    Usage
  • 24
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    1
    • Patent Family Citations
      1
      • Patent Families
        1
  • Usage
    1,358
    • Abstract Views
      1,246
    • Downloads
      112
  • Captures
    24
  • Ratings
    • Download Rank
      502,327

Article Description

Tumor neoantigens are fragments of mutated proteins that contain the mutation, and can be presented by major histocompatibility complex molecules on tumor cells, where they are surveyed by T cells. The rapid and sensitive identification of neoantigen‐specific T cell populations from tumor tissues or blood has proven challenging. A microchip platform for the non‐destructive identification of neoantigen‐specific CD8 T cells is described. The method utilizes a library of neoantigen/MHC tetramers linked to a magnetic nanoparticle via a DNA barcode. The neoantigen‐specificity of the T cells is determined by decoding the barcode through sequential fluorescent microscopy reads. The captured T cells may be further characterized for function, or via matching the neoantigen‐specificity with the T cell receptor gene. Tumor infiltrating lymphocytes and non‐expanded peripheral blood mononuclear cells collected from melanoma patients at various time points across an anti‐PD1 therapy regimen are shown to contain overlapping neoantigen‐specific T cell populations.

Bibliographic Details

Songming Peng; Elizabeth Holman; Xiaozhe Ding; Katherine Guo; Jungwoo Kim; Alexander M. Xu; John E. Heath; Jing Zhou; Yapeng Su; James R. Heath; Jesse M. Zaretsky; Antoni Ribas; Michael T. Bethune; Alice Hsu; Won Jun Noh; David Baltimore; Jami McLaughlin; Donghui Cheng; Owen N. Witte

Elsevier BV

Multidisciplinary

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